RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1 Running title: Endocytic signaling by PECAM-1 cytosolic domain
نویسندگان
چکیده
Carmen Garnacho, Vladimir Shuvaev, Anu Thomas, Lindsey McKenna, Jing Sun, Michael Koval, Steven Albelda, *Vladimir Muzykantov, and *Silvia Muro Dept. of Pharmacology, Targeted Therapeutics Program of the Institute for Translational Medicine and Therapeutics, Institute for Environmental Medicine, Pulmonary Critical Care Division of the Dept. of Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA, Division of Pulmonary, Allergy and Critical Care Medicine, Dept. of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
منابع مشابه
Acute and chronic shear stress differently regulate endothelial internalization of nanocarriers targeted to platelet-endothelial cell adhesion molecule-1.
Intracellular delivery of nanocarriers (NC) is controlled by their design and target cell phenotype, microenvironment, and functional status. Endothelial cells (EC) lining the vascular lumen represent an important target for drug delivery. Endothelium in vivo is constantly or intermittently (as, for example, during ischemia-reperfusion) exposed to blood flow, which influences NC-EC interactions...
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Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a newly assigned member of the Ig immunoreceptor tyrosine-based inhibitory motif superfamily, and its functional role is suggested to be an inhibitory receptor that modulates immunoreceptor tyrosine-based activation motif-dependent signaling cascades. To test whether PECAM-1 is capable of delivering inhibitory signals in B cells an...
متن کاملA novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1.
Antibody conjugates directed against intercellular adhesion molecule (ICAM-1) or platelet-endothelial cell adhesion molecule (PECAM-1) have formed the basis for drug delivery vehicles that are specifically recognized and internalized by endothelial cells. There is increasing evidence that ICAM-1 and PECAM-1 may also play a role in cell scavenger functions and pathogen entry. To define the mecha...
متن کاملPECAM-1 (CD31) regulates a hydrogen peroxide–activated nonselective cation channel in endothelial cells
Hydrogen peroxide (H2O2) released by neutrophils is an important mediator of endothelial cell (EC) injury and vascular inflammation via its effect on EC-free Ca2+, [Ca2+]i. Although the underlying mechanisms are not well understood, platelet endothelial cell adhesion molecule (PECAM)-1/CD-31 is a critical modulator of neutrophil-EC transmigration. PECAM-1 is also known to regulate EC calcium si...
متن کاملPhosphorylation of leukocyte PECAM and its association with detergent-resistant membranes regulate transendothelial migration.
Leukocyte migration across the endothelial lining is a critical step in the body's response to infection and inflammation. The homophilic interaction between endothelial PECAM and leukocyte PECAM is essential for this process. The molecular events that are triggered in the endothelial cell by PECAM engagement have been well characterized; however, the function of leukocyte PECAM remains to be e...
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